https://doi.org/10.4081/acbr.2020.98

A Preliminary estimation of baseline urinary hydroxyproline/creatinine levels in a study population of healthy Nigerians

Vol. 1 No. 1 (2020)
Submitted: 6 August 2020
Accepted: 24 October 2020
Published: 9 November 2020
Biomarkers, urinary, hydroxyproline, creatinine
Abstract Views: 225
PDF: 146
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Chidinma Ifechi Onwuka
Department of Obstetrics and Gynaecology, University of Nigeria Teaching Hospital, Enugu, Nigeria.
Chidozie Onwuka
chidozie.onwuka@unn.edu.ng
https://orcid.org/0000-0001-9509-9581
Department of Oral and Maxillofacial Surgery, University of Nigeria Teaching Hospital, Enugu, Nigeria.
Chibuzor Uguru
Department of Oral and Maxillofacial Surgery, University of Nigeria Teaching Hospital, Enugu, Nigeria.

Background: Hydroxyproline is one of the biochemical markers that can be measured objectively as an indicator of normal biological processes or pathological processes. It is usually raised in disease conditions that are associated with bone resorption.

Aim: To determine the urinary hydroxyproline/creatinine levels in a study population of healthy Nigerians.

Methods: This study recruited 22 consenting participants who served as control for another study at the University of Nigeria Teaching Hospital (UNTH) Ituku-Ozalla, Enugu. All participants were required to fast for at least 12 hours overnight and their early morning second void urine collected between 7am and 8am. The collected urine samples were stored frozen at -20oC until analysis. Colorimetric method of analysis of urinary hydroxyproline and creatinine were done using Biovisionhydroxyproline kit and Randoxcreatinine kit respectively. Bivariate analysis was conducted on the collated data using statistical package of social science (SPSS) version 19. The results were recorded as urinary hydroxyproline alone (μg/μL) and as urinary hydroxyproline/creatinine ratio.

Results: The mean urinary hydroxyproline level of 0.020±0.013μg/μL and urinary hydroxyproline/ creatinine ratio of 0.016±0.006 were noted for healthy Nigerians.

Conclusions: The urinary hydroxyproline levels in the study population of healthy Nigerians are within normal values reported in other healthy populations.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC
Heer M, Baecker N, Milka C, et al. Immobilization induces a very rapid increase in osteoclast activity. Acta Astronaut 2005;57:31-5. DOI: https://doi.org/10.1016/j.actaastro.2004.12.007
Mukhopadhyay M,Sinha R, Pal M, et al. Role of common biochemical markers for the assessment of fracture union. Ind J Clin Biochem 26:274–8. DOI: https://doi.org/10.1007/s12291-011-0143-1
Briot K, Roux C. Biochemical markers of bone remodelling. Gynecol Obstert Fertile 2005;33:1009-13. DOI: https://doi.org/10.1016/j.gyobfe.2005.10.017
Seibel MJ. Biochemical markers of bone turnover, part 1: biochemistry and variability. Clinical Biochem Rev 2005;26:97-122.
Singer FR, Eyre DR. Using biochemical markers of bone turnover in clinical practice. Cleve Clin J Med 2008;75:739-50. DOI: https://doi.org/10.3949/ccjm.75.10.739
Watts NB.Clinical utility of biochemical markers of bone remodelling. Clin Chem 1999;45:1359-68. DOI: https://doi.org/10.1093/clinchem/45.8.1359
Deftos LJ. Bone protein and peptide assays in the diagnosis and management of skeletal disease. Clin Chem 1991;37:1143-8. DOI: https://doi.org/10.1093/clinchem/37.7.1143
Delmas PD. Biochemical markers of bone turnover J Bone Miner Res 1993;2:S549-55. DOI: https://doi.org/10.1002/jbmr.5650081323
Carvo MS, Eyre DR, Gunderg CM. Molecular basis and clinical applications of biological marker of bone turnover. Endocrine Rev 1996;17:333-68.
Ugar DA, KaracaI. Serum osteocalcin as a biochemical bone formation marker. Biochem Archi 1999;15:379-82.
Rissanen J. Markers of bone turnover in preclinical development of drugs for skeletal diseases. PhD Thesis of molecular medicine, Department of Cell Biology and Anatomy. University of Turku, Finland 2013.
Prockop DJ, Kivirikko KI. A relationship of hydroxyproline excretion in urine to collagen metabolism. Ann Intern Med 1967;66:1243-66. DOI: https://doi.org/10.7326/0003-4819-66-6-1243
Kivirikko KI. Urinary excretion of hydroxyproline in health and disease. Int Rev Connect Tissue Res 1970;5:93-163. DOI: https://doi.org/10.1016/B978-0-12-363705-5.50008-7
Krane SM, Munoz AJ, Harris ED. Urinary polypeptides related to collagen synthesis. J Clin Invest 1970;49:716-29. DOI: https://doi.org/10.1172/JCI106284
Ashhurst DE. Collagen systhesized by healing fractures. Clin Orthop 1990;255:273-83.
Krane SM, Kantrowitz FG, Byrne M, et al. Urinary excretion of hydroxylysine and its glycosides as an index of collagen degradation. J Clin Invest 1977;59:819-27. DOI: https://doi.org/10.1172/JCI108704
Indumati V, Patil VS, Jailkhani R. Hospital based preliminary study of osteoporosis in postmenopausal women. Ind J Clin Biochem 2007;22:96-100. DOI: https://doi.org/10.1007/BF02913323
Sarvari BK, Mahader DS, Rupa S, Mastan SA. Study of urine hydroxyproline: creatinine ratio as a bone turnover marker of bone metastases along with some routinely used biochemical parameters in patients with prostate cancer in comparison with bone scintigraphy. Sch J App Med Sci 2013;1:332-8.
Smith SM, Dilon EL, Dekerlagand DE, Davis-Street JE. Variability of collagen cross links: impact of sample collection period. Calcif Tissue Int 2004;74:336-41. DOI: https://doi.org/10.1007/s00223-003-0149-7
Paroni R, De Vecchi E, Fermo I, et al. Urinary hydroxyproline determined with rapid and simple high performance liquid chromatography. Clin Chem 1992;38:407-11. DOI: https://doi.org/10.1093/clinchem/38.3.407
Bowers LD. Kinetic serum creatinine assay. The roles of various factors in determining specificity. Clin Chem Acta 1980;26:551-6. DOI: https://doi.org/10.1093/clinchem/26.5.551
Hodgkinson A, Thompson T. Measurement of Fasting urinary hydroxyproline: creatinine ratio in normal adults and its variation with age and sex. J Clin Pathol 1982;35:807-11. DOI: https://doi.org/10.1136/jcp.35.8.807
George BO. Urinary and anthropometric indices of bone density in healthy Nigerian adults. J Appl Sc Environ Mgt 2003;7:19-23.
Teerlink T, Tavenier P, Netelenbos J. Selective determination of hydroxyproline in urine by high performance liquid chromatography using precolumn derivation. Clin Chem Acta 1989;183:309-11 DOI: https://doi.org/10.1016/0009-8981(89)90365-3
Sachdeva A, Seth S, Khosla AH, Sachdeva S. Study of some common biochemical bone turnover markers in post menopausal women. Ind J Clin Biochem 2005;20:131-4. DOI: https://doi.org/10.1007/BF02893058

How to Cite

Onwuka, C. I., Onwuka, C., & Uguru, C. . (2020). A Preliminary estimation of baseline urinary hydroxyproline/creatinine levels in a study population of healthy Nigerians. Annals of Clinical and Biomedical Research, 1(1). https://doi.org/10.4081/acbr.2020.98

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.